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| Expression levels of microRNA-548b and cancerous inhibitor of protein phosphatase 2A in cervical cancer and precancerous lesions tissues of patients and its correlation with their HPV infection |
| 1. Shijiazhuang Maternal and Child Health Care Hospital, Hebei Province, 050000; 2. Longzhou Hospital of Xingtang, Hebei Province |
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Abstract To investigate the expression levels of microRNA-548b (miR-548b), cancerous inhibitor of protein phosphatase 2A (CIP2A) in cervical cancer (CC) and precancerous lesions tissues of the patients, and to study their correlation with human papillomavirus (HPV) infection of the patients. Methods: From December 2018 to November 2020, 47 patients with CC were selected in group A, 61 patients with cervical precancerous lesions were selected in group B, and 45 patients with cervicitis were selected in group C. The expression levels of miR-548b and CIP2A in cervical tissue were determined by real-time fluorescence quantitative PCR (qRT-PCR). HPV infection was detected by polymerase chain reaction-reverse dot blot. Spearman method was used to analyze the correlation between the expression levels of miR-548b and CIP2A in CC and cervical precancerous lesions tissues and the HPV infection. The correlation between miR-548b expression level in cervical tissues of the patients with cervicitis, in precancerous lesions tissues of the patients, or in cancer tissues of the patients and the CIP2A expression level of the patients was analyzed by Pearson method. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value of miR-548b and CIP2A levels for CC. Results: The expression level of Mir-548b in cervical tissues of the patients with cervicitis, the patients with precancerous lesions, and the patients with CC decreased in turn, while the expression level of CIP2A and HPV positive infection rate of them increased in turn (all P<0.05). There was a negative correlation between the Mir-548b level in the cervical tissues of the patients with CC or the patients with precancerous lesions and their HPV infection, but the CIP2A level was positive correlation with the HPV infection (all P<0.05). The expression of Mir-548b level in the cervical tissues of the patients with cervicitis, the patients with precancerous lesions, and the patients with CC were negatively correlated with their CIP2A level (all P<0.05). The area under the curve (AUC) of Mir-548b level and CIP2A level for diagnosing CC were 0.882 and 0.889, which’s cutoff values were 0.61 and 1.81, which’s sensitivity were 89.4%, and which’s specificity were 72.6% and 78.3%, respectively. The AUC of Mir-548b level combined with CIP2A level for diagnosing CC was 0.945, and which’s sensitivity and specificity were 87.2% and 92.5%, respectively. Conclusion: The expressions of miR-548b in cervical tissue of the patients with CC or precancerous lesions are low, but the CIP2A expressions are high. Both of them are significantly associated with HPV infection. The combination of miR-548b and CIP2A levels has better diagnostic efficiency for CC.
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