To explore the relationship between human papilloma virus (HPV) infection of pregnant women with condyloma acuminatum and their pregnancy outcomes, and to study the changes of the Th1/Th2 cytokines levels in peripheral blood of the pregnant women. Methods: 122 pregnant women with condyloma acuminatum from February 2010 to October 2020 in observation group were analyzed retrospectively. 121 healthy pregnant women during the same period were selected in control group. The serum Th1/Th2 cytokine level, the delivery method, and the pregnancy outcomes of the women in the two groups were analyzed. Results: In the observation group, there were 77.1% women with HPV gene high-risk subtype infection, 13.1% women with HPV low-risk subtype infection, and 9.8% women with HPV mixed subtype infection. In the observation group, there were 30.3% newborns with HPV gene high-risk subtype infection, 4.9% newborns with HPV low-risk subtype infection, and 3.3% newborns with HPV mixed subtype infection. The rates of preterm delivery, premature rupture of membranes, respiratory distress, fetal growth restriction, and postpartum hemorrhage, and the levels of Th1 and Th1/Th2 of the women in the observation group were significant lower than those of the women in the control group, but the Th2 level of the women in the observation group was significant higher (all P<0.05). There were no significant differences in the delivery model, and maternal and infant outcomes, and Th1 / Th2 cytokines levels among the women with different HPV subtype infection (P>0.05). Conclusion: Pregnant women with condyloma acuminatum because of HPV infection has obvious Th1/Th2 immune imbalance, which will affect pregnancy outcomes, but Th1/Th2 immune imbalance situation and pregnancy outcomes are not affected by different HPV subtypes.