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| Clinical effect of biopsy methods of different blastocyst |
| Shijiazhuang Obstetrics and Gynecology Hospital Affiliated to Hebei Medical University, Shijiazhuang，Hebei Province, 050011 |
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Abstract To compare the clinical effect of two blastocyst biopsy protocols used in preimplantation genetic testing (PGT). Methods: A retrospective analysis was conducted on the clinical data of the women who had received PGT from January 2019 to June 2020. The outcomes of the women were compared between group A and group B. The blastocyst of the women in group A was punch biopsy on the 3rd day (D3) and the blastocyst of the women in group B were punch hatching punch on the 5th day (D5)combined with the shrinkage blastocyst were biopsy on the 6th (D6). Results: The average age of these woman was 30.08±3.63 years old. There were 75 PGT cycles, which included 63 cycles of the women with chromosomal diseases, 6 cycles of the women with monogenic diseases, and 6 cycles of the women with recurrent miscarriage. 348 blastocysts were biopsied, and 339 were successfully biopsied with the biopsy success rate of 97.4%. The rate of normal blastocysts detected by PGT was 41.9% (142/339). There were 51 cycles of blastocysts transplantation in 45 women, and which clinical pregnancy rate was 64.7% (33/51). The blastocyst formation rate (64.8%) of the women in group A was significant lower than that (72.0%) of the women in group B (P=0.034), and the available blastocyst formation rate (63.7%) of the women in group A was significant lower than that (73.0%) of the women in group B (P=0.025). There were no significant differences in D3 optimal embryo rate, D3 available embryo rate, and clinical pregnancy rate of the women between the two groups. Conclusion: Compared with those of the method of D3 punch biopsy of blastocyst, the blastocyst formation rate and the usable blastocyst rate of the method of D5 punch hatching of blastocyst combined with D6 shrinkage biopsy of blastocyst are better.
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