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| Effects of miR-802 targeting STAT6 on proliferation, apoptosis, invasion， and migration of ovarian cancer cells |
| Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan Province, 637000 |
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Abstract To explore the effect of miR-802 on the proliferation, apoptosis, migration and invasion of ovarian cancer cells. Methods: The cancer tissue and paracancerous tissues samples of 63 patients with ovarian cancer who underwent surgical treatment and were pathologically confirmed from July 2016 to July 2019 were collected. RT-qPCR was used to detect the expression of miR-802 in ovarian cancer tissues, corresponding adjacent tissues, human ovarian surface epithelial cells HOSE, and ovarian cancer cells SKOV-3, A2780, and HO8910. The relationship between the low expression of miR-802 and the clinical parameters of patients with ovarian cancer was analyzed. The sKOV-3 ovarian cancer cells with the lowest expression of miR-802 were selected for subsequent experiments. SKOV-3 cells were transfected into miR-802 mimic by lipofectamineTM2000 were in miR-802 mimic group, and SKOV-3 cells were transfected into mimic NC by lipofectamineTM2000 were in mimic NC group, and SKOV-3 cells were not given treatment were in control group. RT-qPCR was used to detect the transfection efficiency and STAT6 mRNA expression level, CCK8 test was used to detect the proliferation of the cells in the three groups, Transwell test was used to detect the migration and invasion of the cells in the three groups, flow cytometry was used to detect the apoptosis of the cells in the three groups, dual-Luciferase test was used to verify the targeting relationship between miR-802 and STAT6, and western-blot test was used to detect the expression levels of STAT6, Bax, Ki-67, and MMP-2 Protein of the cells in the three groups. Results: The expression of miR-802 in cancer tissues (0.51±0.18) was significant lower than that (1.49±0.22) in paracancerous tissues, and the low expression of miR-802 was associated with FIGO stage and lymph node metastasis (all P<0.05). Compared with that of human ovarian epithelial cells HOSE, the expression of miR-802 in all ovarian cancer cells was significant lower. Compared with those in mimic NC group, the expression level of miR-802 in miR-802 mimic group was significant higher, and the transfection efficiency was significant better. Compared with those in the control group and mimic NC group, the cell viability, the Ki-67 protein level, migration and invasion ability of the cells, and the MMP-2 protein level in miR-802 mimic group were significant lower, but the apoptosis rate and the Bax protein expression level were significant higher (all P<0.05). Dual-luciferase assay showed that miR-802 reduced wild-type STAT6 3’UTR luciferase activity significantly, but had no effect on mutant STAT6 3’UTR luciferase activity. Compared with those in the control group and mimic NC group, the mRNA and protein expressions of STAT6 in miR-802 mimic group were significant lower (P<0.05). Conclusion: The expression of miR-802 in ovarian cancer tissues was lower. Overexpression of miR-802 can inhibit the proliferation, migration, and invasion of ovarian cancer cells, and promote apoptosis of cancer cells, which may play a role by targeting STAT6.
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