To detect the expression of Netrin-1, DCC, uncoordinated homologous gene homolog-5 B (BUNC5B), and vascular endothelial growth factor (VEGF) in the villi tissues of women with missed abortion. Methods: 60 pregnant women were included and were divided in two groups from February 2020 to August 2020. Among them, 30 normal pregnant women who had wanted induced abortion were in control group, and the other 30 women who had accepted induced abortion because of missed abortion were in research group. Real-time fluorescent quantitative polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used to detect the mRNA and protein levels of Netrin-1, DCC, UNC5B, VEGF in the villi tissues of the women in the two groups. Results: The results showed that the mRNA relative expression levels of Neterin-1, DCC and VEGF in the villi tissue of the women in the research group were significant lower than those of the women in the control group, but the UNC5B mRNA level in the villi tissue of the women in the research group was significant higher (P＜0.05). The relative expression levels of netrin-1 (0.78±0.22), DCC (0.52±0.15) and VEGF (0.81±0.23) of the women in the research group were significant lower than those (1.24±0.29, 09±0.25, and 1.42±0.35) of the women in the control group (P＜0.05). The UNC5B protein level (1.82±0.34) of the women in the research group was significant higher than that (1.04±0.21) of the control group (P＜0.05). Conclusion: The mRNA and protein expressions of Netrin-1, DCC，and VEGF in the villi tissue of the women with missed abortion are all significant lower than that of the women with normal pregnancy, but the mRNA and protein expressions of UNC5B in the villi tissue of the women with missed abortion are all significant higher, which abnormal expression may be related to the obstacle of the villi blood vessels formation of the women with missed abortion.