|
|
|
| Relationship between miR-124 and IGFBP-3 levels of obese women with polycystic ovary syndrome and their glucose and lipid metabolism |
| Fuyuan NO.1 Hospital, Yiwu, Zhejiang Province, 32220 |
|
|
|
|
Abstract To investigate the relationship between serum miR-124, insulin-like growth factor binding protein-3 (IGFBP-3) expression levels of obese women with polycystic ovary syndrome (PCOS) and their glucose and lipid metabolism. Methods: From February 2019 to February 2020, 90 obese women with PCOS were selected in group A, and 52 women with PCOS and normal weight were selected in group B, another 40 healthy women were selected in group C during the same period. The levels of fasting blood glucose (FPG), triglyceride (TG), serum total cholesterol (TC), low density lipoprotein (LDL), high density lipoprotein (HDL) and fasting insulin (FINS) of the women in the three groups were detected by biochemical analyzer, and their HOMA insulin-resistance (HOMA-IR) was calculated. Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression level of serum miR-124, and the expression level of serum IGFBP-3 was detected by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between the levels of miR-124 and IGFBP-3 of the obese women with PCOS and their glucose and lipid metabolism. The influence factors were analyzed. Results: Compared with those of the women in group C and B, the IGFBP-3 level and glucose and lipid metabolism indexes, such as FPG level, TG level, TC level, LDL level, FINS level, and HOMA-IR value of the women in group A were significant higher, while miR-124 and HDL levels were significant lower (all P＜0.05). There were no significant differences in the levels of IGFBP-3 and miR-124, and the values of glucose and lipid metabolism indexes of the women between group C and group B. The expression of miR-124 was negatively correlated with the levels of FPG, TG, TC, LDL, and FINS, and HOMA-IR value, but which was positively correlated with HDL level (P＜0.05). The IGFBP-3 level was positively correlated with the levels of FPG, TG, TC, LDL, and FINS, and HOMA-IR value, but which was negatively correlated with HDL level (P＜0.05). Multiple linear regression analysis showed that TG level of obese women with PCOS was the main factor affecting the expression of serum miR-124 level. The FPG and FINS levels, and HOMA-IR value were the main factors affecting the expression of their serum IGFBP-3 level. Conclusion: The serum levels of miR-124 and IGFBP-3 of obese women with PCOS are closely related to their lipid metabolism. The detection of serum miR-124 and IGFBP-3 may provide reference for clinical diagnosis and treatment of PCOS.
|
|
|
|
|
|
|
|
|
