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| Expressions of LncRNA NEAT1 and TLR2 in peripheral blood mononuclear cell of pregnant women with unexplained recurrent spontaneous abortion |
| 1.Tunchang People's Hospital, Tunchang, Hainan Province, 571600；2. Hainan People's Hospital, Hainan Affiliated Hospital of Medical College |
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Abstract To investigate the expressions of lncRNA NEAT1 and TLR2 in peripheral blood mononuclear cell (PBMC) of pregnant women with unexplained recurrent spontaneous abortion (URSA). Methods: The clinical data of pregnant women from May 2016 to March 2018 were analyzed retrospectively, which included 97 women with URSA in group A, 50 women with normal pregnancy who wanted induced abortion in group B, and 54 healthy non-pregnant women in group C. The expression levels of lncRNA NEAT1, TLR2mRNA, and TLR2 protein in PBMC, and the levels of serum TNF-α, IL-1β, and IL-6 of the women in the three groups were detected. Pearson correlation analysis was used to analyze the correlations between lncRNA NEAT1 level of the women with URSA and their TLR2, TNF-α, IL-1β and IL-6 levels. ROC curve was used to analyze the diagnostic values of lncRNA NEAT1 and TLR2 mRNA levels for URSA. Logisitic regression analysis was used to analyze the influencing factors of URSA. Results: Compared to those of the women in group C and B, the levels of lncRNA NEAT1 and mRNA and protein of TLR2 in PBMC of the women in group A were significant higher. The serum TNF-α level, the IL-1β level, and the IL-6 level of the women in group A were 19.73±4.47pg/ml, 7.51±1.01pg/ml, and 25.39±5.47pg/ml, which were all significant higher than those (9.31±2.57pg/ml, 2.39±0.54pg/ml, and 12.15±3.54pg/ml) of the women in group B, and those (10.24±3.15pg/ml, 2.58±0.59pg/ml, and 13.52±3.76pg/ml) of the women in group C (P＜0.05). In group A, lncRNA NEAT1 expression of the women was positively correlated with their expression levels of TLR2 mRNA, TNF-α, IL-1β and IL-6 (P＜0.05). Both lncRNA NEAT1 and TLR2 were the influencing factors of URSA. The sensitivity, the specificity, and area under curve of lncRNA NEAT1 level combined with TLR2 level for diagnosing URSA were 93.6%, 90.4%, and 0.906, which all were the highest (P＜0.05). Conclusion: lncRNA NEAT1 may be involved in the occurrence of URSA recurrent by regulating the expression of downstream related inflammatory factors of TLR2 signaling pathway, which is a potential therapeutic target.
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