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Abstract To explore the influencing factors of the effect of oocyte resuscitation of mature oocytes cryopreservation in infertile women, and to provide evidences for clinical decision-making. Methods: The clinical data of 2691 mature oocytes from the women with infertile caused by their husbands were retrospectively analyzed. According to the survival situation after oocyte resuscitation, these cryopreservation oocytes were divided into 2003 survival oocytes and 688 non-survival oocytes. According to age, these oocytes of the women were divided 2297 oocytes of the women <35 years old and 394 oocytes of the women ≥35 years old. Univariate and multivariate logistic regression analysis were applied to explore the risk factors of affecting oocyte thawing. Results: The survival rate of cryopreservation oocytes after resuscitation was 74.4% (2003/2691). Advanced age (OR=0.94, 95%CI 0.91-0.97), less antral follicles (OR=1.03, 95%CI 1.01-1.04), and endometriosis (OR=0.68, 95%CI 0.49-0.95) were the independent risk factors affecting oocyte thawing of women under 35 years old. For women ≥35 years old, basal estradiol level (OR=0.98, 95%CI 0.98-0.99), prolonged cryopreservation time (OR=0.98, 95%CI 0.97-0.99), endometriosis (OR=0.15, 95%CI 0.06-0.36), and secondary infertility (OR=0.22, 95%CI 0.13-0.37) were the independent risk factors affecting oocyte thawing. Conclusion: Age is the key factor affecting the resuscitation of human mature oocytes after cryopreservation. The factors affecting the resuscitation of human mature oocytes after cryopreservation of the women with different ages are different. Before 35 years old, the risk of oocyte thawing failure of thewomen increased significantly with the increase of age, with the decrease of antral follicles, and with the occurrence of endometriosis. For women≥35 years old, elevated basal estradiol level, prolonged cryopreservation time, and endometriosis were the key risk factors for oocyte thawing failure.
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