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| Effect of PTCH1 gene on the proliferation and apoptosis of ovarian cancer cells through the Hedgehog signaling pathway |
| Huangshi Aikang Hospital, Huangshi, Hubei Province, 435000 |
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Abstract To explore the effect of PTCH1 gene on the proliferation and apoptosis of ovarian cancer cells through the Hedgehog signaling pathway. Methods: RT-qPCR technology was used to detect the expression of PTCH1 gene in ovarian cancer tissues of 80 patients with ovarian cancer and in normal ovarian tissues of 80 women, in human ovarian cancer cell lines SKOV3 and A2780, and in human normal ovarian epithelial cell line IOSE80. The stable growth SKOV3 ovarian cancer cells were selected and were randomly divide into pIRES2-Ptch1 group, pIRES2-NC group, and pIRES2-Control group. The overexpression plasmid vector pIRES2-Ptch and the nonsense sequence pIRES2-NC were transfected into SKOV3 ovarian cancer cells, respectively. The cells in the control group were given nothing. RT-qPCR technology was used to detect the expression of PTCH1 gene in cells, and MTT test and flow cytometry were used to detect the proliferation and apoptosis of SKOV3 ovarian cancer cells. Results: The expression level of PTCH1（0.332±0.041）, and the positive rate of PTCH1 protein （23.8%） in ovarian cancer tissues were significantly lower than those (0.701±0.052 and 52.5%) in normal ovarian tissues (P<0.05). The expression levels of PTCH1 mRNA and PTCH1 protein in SKOV3 and A2780 ovarian cancer cells were significantly lower than those in IOSE80 normal ovarian epithelial cells (P<0.05). The expression level of PTCH1 in SKOV3 ovarian cancer cells in the pIRES2-Ptch1 group was significantly higher than that in the pIRES2-Scramble group and the control group (P<0.05). The proliferation activity of SKOV3 ovarian cancer cells transfected by PTCH1 gene overexpression had decreased in pIRES2-Ptch1 group, but which had increased in pires2-scramble group and in the blank control group (P<0.05). The apoptosis rate (16.22±1.08%) of the cells in piRES2-PTCH1 group was significantly higher than that (5.62±0.34%) of the cells in iRES2-scramble group and that (5.49±0.29%) of the cells in blank control group (P<0.05). Conclusion: PTCH1 gene can inhibit the expression of transcription factor Gli1 and the proliferation of ovarian cancer cells, and can induce the apoptosis of ovarian cancer cells by activating the Hedgehog signaling pathway. PTCH1 gene may be a potential diagnosis and treatment target of epithelial ovarian cancer.
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