To explore the levels of serum albumin(HSA) and integrin β1(ITGβ-1) of pregnant women with preeclampsia(PE), and to study its diagnostic significance for PE. Methods: 98 pregnant women with PE from January 2018 to December 2020 were analyzed retrospectively, and these women were divided into group A(34 women with severe PE) and group B(63 women with mild PE) according to the severity of PE. And another 35 normal pregnant women were selected in group C. The levels of serum HSA and ITGβ-1 of the women in the three groups were detected, and the levels of serum HSA and ITGβ-1, and their relevant to  the severity of PE, and the value of the levels of serum HSA and ITG for diagnosing PE were analyzed. Results: The blood pressure value and the serum creatinine level, 24h urinary protein value, and serum ITGβ-1 level of the women in group A and B before delivery were significantly higher than those of the women in group C, while the gestational weeks and serum HSA level of the women in group A and B were significantly lower than those of the women in group C, and the change ranges of which of the women in group A were significant more than those of the women in group B(all P<0.05). Pearson correlation analysis showed that serum ITGβ-1 level of the women in group A and B was positively correlated with their blood pressure(r=0.695, 0.710, P<0.05), but the serum HSA level of the women in group A and B was negatively correlated with their blood pressure(r=-0.772, -0.704, P<0.05). ROC curve of the serum HSA and ITGβ-1 for diagnosing PE was established, and the AUC of the serum HSA and ITGβ-1 for diagnosing PE was 0.736 and 0.731, respectively. The AUC of the combination of serum HSA and ITGβ-1 for diagnosing PE was 0.814, which was significantly higher than that of the serum HAS level or ITGβ-1 level alone(P<0.05). Conclusion: The serum HSA level of the pregnant women with PE increases, but the ITGβ-1 level of them decreases, both of which are involved in the occurrence and development of PE, so they have certain clinical reference value for diagnosing PE.