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| Expression of microRNA-183 in ovarian tissue and its effect on the proliferation and apoptosis of ovarian cancer cells |
| 1.Zhangjiakou Maternal and Child Health Care Hospital, Hebei Province, 075000; 2.Beijing Shijitan Hospital |
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Abstract To investigate the expression of microRNA-183 in ovarian cancer cells, and to study its effect on the proliferation and apoptosis of ovarian cancer cells.Methods: Fluorescence quantitative real-time PCR(RT-QPCR) was used to detect the expression level of Mir-183 gene in ovarian cancer and paracancer tissues.The proliferation of human ovarian cancer cells(SKOV3) and human ovarian cancer ovarian cancer cell(OVCAR3) lines introduced by Mir-183 simulant was determined by the thiazolam(MTT) assay.Cell apoptosis was detected by flow cytometry.Results: The mir-183 expression level(2.75±1.02) in ovarian cancer tissue was significant lower than that(5.43±1.27) in paracancer tissue(P<0.05).MTT and flow cytometry results showed that compared with that of cells without transfection, the proliferative activity of ovarian cancer cells after transfection had decreased significantly(P<0.05）, but the apoptosis rates of SKOV3 and OVCAR3(18.13±1.85% and 21.11±2.16%) had increased significantly(P<0.05).Conclusion: The expression level of microRNA-183 is closely related to the occurrence of ovarian cancer, and the high expression level of microRNA-183 can inhibit the proliferation of ovarian cancer cells and promote the apoptosis of ovarian cancer cells.
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