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| Analysis of the expression of local regulatory T lymphocytes in the cervix of patients with persistent human papilloma virus infection |
| Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Zhejiang Province, 325000 |
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Abstract To analyze the relationship between persistent human papillomavirus (HPV) infection of the patients and their expression of local regulatory T lymphocytes (Treg) in the cervix. Methods: A total of 476 patients with HPV positive were selected as the research subjects from May 2018 to October 2020. According to the results of followed up, these patients were divided into group A (178 patients with persistent HPV infection) and group B (298 patients with HPV turning to negative). The expression of Treg was detected by flow cytometry, and HPV-DNA was detected by hybridization capture 2 generation (HC-Ⅱ). Results: The expression of Treg (8.2±2.0%) of the patients in group A was significant higher than that (6.1±3.8 %) of the patients in group B (P＜0.05). In group A, the expression of Treg of the patients with ≥35 years old, the patients with multiple HPV infection, or the patients with low grade squamous intraepithelial neoplasia (LSIL) by cervical biopsy was significant higher (P＜0.05), but which had no significant difference between the patients with difference (≥24kg/m2 or <24kg/m2) of body mass index (P＞0.05). Age ≥35 years old, multiple HPV infection, and Treg≥7.8% of the patients were the independent risk factors of their persistent HPV infection (P＜0.05).The expression of Treg for persistent HPV infection had predictive efficacy (P＜0.001), and the AUC, the specificity, and the sensitivity of which were 0.834 (95%CI: 0.754-0.914), 69.1%, and 73.4%, respectively. Conclusion: The persistent infection of HPV of the patients is related to the immune microenvironment of their cervix, and local Treg expression in the cervix is valuable for predicting the persistent infection of HPV.
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