To investigate the influence of KIF14 gene on proliferation, apoptosis, and cisplatin sensitivity of endometrial carcinoma cells with cisplatin resistant. Methods: The expression levels of KIF14 in endometrial carcinoma cell line Ishikawa and cisplatin resistant cell line Ishikawa/DDP were detected by RT-qPCR and Western-blot. The Ishikawa/DDP cells in siRNA-KIF14 group were transfected by siRNA-KIF14 and the Ishikawa/DDP cells in siRNA-NC group were transfected by siRNA-NC, and the cells in drug-resistant group were not treated. The proliferation of cisplatin resistant cells of endometrial carcinoma was detected by cloning formation experiment after interfering KIF14. Flow cytometry was used to detect the apoptosis of cisplatin resistant cells in endometrial carcinoma after interfering KIF14, and western-blot was used to detect the expression levels of proliferation, apoptosis, and drug resistant gene related proteins, such as ki-67, Bax, cleaved Caspase-3, MDR1, and P-gp. The cells in each group were added to cisplatin with series of concentrations, and the sensitivity of cisplatin of cisplatin resistant cells in endometrial carcinoma was detected by MTT assay after interfering KIF14. Results: KIF14 was expressed highly in cisplatin-resistant endometrial cancer cell line Ishikawa/DDP compared with Ishikawa (P＜0.001). Compared with those of the cells in drug-resistant group and si-NC group, the number of Ishikawa/DDP cell clone formation and ki-67 protein level in si-KIF14 group had decreased, and the cell apoptosis rate and the levels of Bax and Cleaved caspase-3 protein had increased, but the IC50 value had decreased after interfering KIF14. The levels of MDR1 and P-GP protein of the cells in siKIF14 group had decreased (all P＜0.001). Conclusion: Interfering KIF14 expression can inhibit the proliferation of Ishikawa/DDP of the endometrial carcinoma cells with platin resistant, which can promote apoptosis of the cells and increase their sensitivity to cisplatin.