|
|
|
| Expression of FOXP3 mRNA and RORγt mRNA in peripheral blood of women with recurrent spontaneous abortion and their diagnostic value |
| Fushun Mining General Hospital, Liaoning Health Industry Group， Liaoning Province, 113000 |
|
|
|
|
Abstract To explore the expression of FOXP3 mRNA and RORγt mRNA in peripheral blood of women with recurrent spontaneous abortion (RSA), and to study their diagnostic efficiency in clinical practice. Methods: From January 2018 to December 2019, 106 women with RSA were selected in group A, 39 healthy early pregnant women were selected in group B, and 42 healthy women were selected in group C. Real-time fluorescence PCR was used to determine the levels of FOXP3 mRNA and RORγtmRNA in peripheral blood of women in the three groups. Enzyme-linked immunosorbent assay was used to detect the levels of interleukin-6 and 7 (IL-6 and IL-7), and human thymus activation regulatory chemokine 17 (CCL17), CC chemokine receptor 4 (CCR4) of women in the three groups. ROC curves was drawn to analyze the value of FOXP3 mRNA and RORγt mRNA levels for diagnosing RSA. Results: The FOXP3 mRNA level of women in group A was significant higher than that of women in group C, but was significant lower than that of women in group B. The levels of RORγtmRNA, IL-6, and IL-7 of women in group A were significant higher than those of women in group B and C. The CCL17 and CCR4 levels of women in group A were significant lower than those of women in group C, but were significant higher than those of women in group B (all P＜0.05). ROC curve analysis showed that the area under the curve, the sensitivity, the specificity, and the accuracy of FOXP3mRNA level combined with RORγtmRNA level for diagnosing women with RSA were 0.962， 94.34%, and 92.18%, and 100%, which were all significant higher than those of FOXP3 mRNA level or RORγtmRNA level alone (P＜0.05). Conclusion: The abnormal expression of FOXP3 mRNA and RORγt mRNA in peripheral blood of women with RSA may be related to their immune disorders. The detection of FOXP3mRNA level combined with RORγtmRNA level has higher value for diagnosing RSA.
|
|
|
|
|
|
|
|
|
