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| Value of cervical biopsy by colposcopy combined with EGFR, P16, and P53 protein detections for diagnosing patients with cervical lesions |
1. Graduate School of Xuzhou Medical University, Jiangsu Province, 201300；2. Xuzhou Oncology Hospital;
3. Affiliated Hospital of Xuzhou Medical University |
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Abstract To investigate the value of colposcopy biopsy combined with epidermal cell growth factor (EGFR), P16, and P53 protein detection of patients with abnormal cervical neoboehner's technique (TCT) for diagnosing the staging of cervical intraepithelial neoplasia (CIN) and cervical cancer. Methods: 450 patients with abnormal TCT results were included from March 2016 to June 2019. Among them, 112 patients in study group were confirmed CIN by colposcopy and pathological biopsy, and other 60 patients in control group with normal cervical tissue. The results of colposcopy and pathological examination of patients were compared between the two groups. And the expression levels of EGFR, P16 and P53 protein of patients in the two groups were detected by immunohistochemistry. Results: In the study group, the pathological biopsy confirmed that there were 8(7.1%) patients with chronic cervicitis, 36(32.1%) patients with CIN I, 32(28.6%) patients with CIN II, 20(17.9%) patients with CIN III, and 16(14.3%) patients with cervical squamous carcinoma. The coincidence rates of the colposcopy and the pathological biopsy for diagnosing chronic cervicitis, CIN I, CIN II, CIN III, and cervical squamous carcinoma were 100.0%, 94.4%, 90.6%, 85.0% and 100.0%, respectively. With the escalation of cervical lesions, the expression levels of EGFR, P16 and P53 proteins of the patients increased gradually, which were not expressed in the normal cervical epithelium (P＜0.05). There were 11 (68.8%) patients with cervical cancer stage I and 5(31.3%) patients with cervical cancer stage II, and there were significant different in expressions of EGFR, P16 and P53 protein of the patients with different stages of cervical cancer (P＜0.05). Conclusion: Colposcopy biopsy can effectively improve the accuracy rate for diagnosing cervical disease of patients with abnormal TCT. And the colposcopy biopsy combined with the expression of EGFR, P16, and P53 proteins levels has clinical significance to distinguish low grade and high grade of cervical intraepithelial neoplasia of patients.
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