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| The correlation between the expressions of miR-543 and miR-135a and the recurrence of uterine adhesions of women after hysteroscopic treatment |
| People's Hospital of Jingjiang, Jiangsu Province, 214500 |
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Abstract To investigate the correlation between the expressions of miR-543 and miR-135a and the recurrence of uterine adhesions of women after hysteroscopic treatment. Methods: From September 2017 to September 2019, 78 women with moderate and severe uterine adhesions treated by hysteroscopy were selected and were divided into group A (23 women with recurrence within 1 year after hysteroscopic treatment) and group B (55 women without recurrence). The expression levels of miR-543 and miR-135a were detected by real-time fluorescent quantitative PCR (qRT-PCR), Pearson's method was used to analyze the correlations between the levels of miR-543 and miR-135a and SIRT1 mRNA, TGF-β mRNA expressions, Logistic regression model was used to analyze the risk factors of recurrence of uterine adhesions after hysteroscopic treatment, and ROC curve was used to analyze the values of the expression levels of miR-543 and miR-135a for predicting recurrence of uterine adhesions after hysteroscopic treatment. Results: The proportions of severe adhesion and preoperative hypomenorrhea, and preoperative of amenorrhea, the number of preoperative uterine cavity operation, and the expression levels of SIRT1 mRNA and TGF-β mRNA of women in group A were significant higher than those of women in group B, while the expression levels of miR-543 and miR-135a of women in group A were significant lower (all P＜0.05). Both Mir-543 level and Mir-135a level were negatively correlated with SIRT1 mRNA and TGF- mRNA levels (P＜0.05). The proportion of severe adhesion, the number of preoperative uterine cavity operation, the high levels of SIRT1 mRNA and TGF-β mRNA, and the low miR-543 and Mir-135a levels were independent risk factors of recurrence after endoscopic treatment (P＜0.05). The area under the curve, the sensitivity, and the specificity of combined detection of miR-543 and miR-135a for predicting the recurrence after endoscopic treatment were 0.856 (95%CI: 0.771-0.924), 91.3%, and 74.5%, respectively. Conclusion: The decreasing of the expression levels of miR-543 and miR-135a in endometrial tissue of women with recurrence of uterine adhesions has certain value for evaluating the recurrence of women with recurrence of uterine adhesions after endoscopic treatment.
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