To analyze the correlation between the levels of inhibin B (INH-B), leptin (Leptin), and anti-Mullerian hormone (AMH) of patients with polycystic ovary syndrome (PCOS) and their sex hormones levels. Methods: 120 patients with PCOS were selected in study group from January 2018 to January 2019. Another 120 healthy women who underwent physical examination were selected in control group during the same period. The levels of INH-B, Leptin, AMH and sex hormones of the women in both groups were detected, which’s correlation with the obesity and the disease classification of patients with PCOS was analyzed. Pearson correlation analysis was used to analyze the relationship between the levels of INH-B, Leptin, AMH, INH-B, Leptin, AMH and sex hormones levels. Results: The serum levels of INH-B, Leptin, AMH, testosterone(T) and luteinizing hormone (LH) of women in the study group were significant higher than those of women in the control group, but the level of FSH was significant lower than that of women in the control group (P<0.05). In the study group, the levels of Leptin, AMH, and T of women were positively correlated with their obesity, but the INH-B and LH levels were negatively correlated with their obesity (P<0.05), and the FSH level was not correlated with obesity (P>0.05). The levels of serum INH-B, Leptin, AMH, T and LH were positively correlated with the severity of the disease (P<0.05). The INH-B level was positively correlated with the levelsof Leptin and AMH (r=0.328, 0.236, P=0.016, 0.028), but the Leptin level was positively correlated with the AMH level (r=0.685, P=0.002). The levels of INH-B, Leptin, and AMH were positively correlated with the levels of T and LH, but were negatively correlated with the FSH level (P<0.05). Conclusion: INH-B, Leptin and AMH of patients with PCOS are involved in the occurrence and development of PCOS, which are related to abnormal sex hormones levels, so they maybe used as predictors for detecting PCOS.