|
|
|
| The expressions of miR-34a and miR-199a in cervical lesions tissue and their correlations with high risk HPV infection |
| Huizhou Hospital of Guangzhou University of Traditional Chinese Medicine（Huizhou Traditional Chinese Medicine Hospital）, Guangdong Province, 516003 |
|
|
|
|
Abstract To investigate the expressions of microRNA-34a (miR-34a) and microRNA-199a (miR-199a) in cervical lesions tissue, and to study their relationships with highrisk human papillomavirus (HPV) infection. Methods: From March 2016 to November 2018, 306 women who were screened for cervical lesions were selected as the study objects, and they all were examined by thin layer liquid based cytology (TCT) and HPV-DNA. Among them, the women with TCT positive were given colposcopy histopathological biopsy, and were divided into high-risk HPV positive group and negative group according to the result of HPV-DNA test. The expression of miR-34a and miR-199a in cervical abscission cells was detected by SYBR Green fluorescence quantitative PCR, and the expression of miR-34a and miR-199a, and the rate of high-risk HPV infection were compared among these groups. Results: Pathological results showed that 45 women with cervical cancer (in group A), 111 women with cervical intraepithelial neoplasia (CIN) (42 women with CIN Ⅰin group B1, 33 women with CIN Ⅱin group B2, and 36 women with CIN Ⅲ in group B3), and 150 women with normal cervix or cervical chronic inflammation (in group C). There were 87 women with high-risk type HPV infection, and the positive rate of HPV infection of women in group C, group B1, group B2, group B3, and group A had increased in turn (P<0.05). The detection rate of HPV16 infection was the highest in the women with high-risk HPV positive infection, followed by HPV18. the expressions of miR-34a and miR-199a in cervical exfoliated cells of women in group C, group B1, group B2, group B3, and group A had decreased in turn, and the expressions of miR-34a and miR-199a of women with high-risk HPV positive infection were significant lower than those of women without HPV infection (P<0.05). Pearson correlation analysis showed that the expressions of miR-34a and miR-199a in cervical exfoliated cells of women with cervical lesions were negatively correlated with CIN classification and high-risk HPV infection (P<0.05). Conclusion: With the aggravation of cervical lesions, the expressions of miR-34a and miR-199a in cervical exfoliated cells decrease and the infection rate of high-risk HPV increase, the expressions of miR-34a and miR-199a are negatively correlated with the infection rate of high-risk HPV. The detection rate of HPV16 infection is the highest. The expressions of miR-34a and miR-199a can be effective indicator for early screening of cervical cancer.
|
|
|
|
|
|
|
|
|
