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| Clinical value of amniotic fluid cell culture combined with FISH for diagnosing fetal chromosomal abnormalities |
| Henan Maternal and Child Health Care Hospital (The Third Affiliated Hospital of Zhengzhou University), Henan Province, 450052 |
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Abstract Objective: To analyze the clinical value of amniotic fluid cell culture combined with fluorescence in situ hybridization (FISH) for diagnosing fetal chromosomal abnormalities of pregnant women during the second and third trimesters of pregnancy. Methods: 2904 pregnant women during the second or third trimesters of pregnancy were selected, and their fetal chromosomal abnormalities were diagnosed by amniotic fluid cell culture combined with FISH. The diagnostic results were analyzed, and the advantages and disadvantages of the two diagnostic methods were summarized. Results: Of 2904 amniotic fluid specimens, 2815 (96.9%) were successfully cultured. The samples after inoculation and cultured were divided into two groups for karyotype analysis. The results of chromosome analysis of 2815 amniotic fluid specimens in the two groups were identical. Among 2815 successfully cultured specimens, 139 cases (4.9%) were found to have abnormal karyotypes. FISH was successfully detected in 2904 amniotic fluid samples with a success rate of 100.0%. FISH found 90 cases with chromosome abnormality, which was consistent with that result by amniotic fluid cell karyotype analysis. The other 3 cases with chromosomal deletion and duplication, 44 cases with structural abnormalities, and 2 cases with chimeras detected by karyotype were not detected by FISH. Conclusion: The success rate of amniotic fluid cell culture of women during the second or third trimesters of pregnancy is worthy of affirmation, which combined with FISH can achieve rapid and early diagnosis of chromosome number abnormalities, so it is a beneficial complement to traditional cytogenetic methods.
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