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| Effects of different dosage of semiaquilegia granule on the levels of serum sex hormones, VEGF and ITG-β3, and embryo implantation rate of rats with bad endometrial receptivity because of kidney-deficiency blood-stasis type |
| 1. Southwestern Medical University, Luzhou, Sichuan Province, 646000; 2. Hospital of Traditional Chinese Medicine Affiliated to Southwestern Medical University |
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Abstract Objective: To study effect of different doses of semiaquilegia granule (SG) on the levels serum sex hormones, vascular endothelial growth factor (VEGF), and integrin-β3 (ITG-β3), and rate of embryo implantation of rats with bad endometrial receptivity because of kidney-deficiency blood-stasis type. Methods: 96 SPF class mature female SD rats were randomly divided into 6 groups (16 rats in each group). The rats in group A were blank control, and the rats in group B, C, D, E and F were model rats with bad endometrial receptivity because of kidney-deficiency blood-stasis type. The rats in group B, C and D were administered with 0.5 mL traditional Chinese medicine (TCM) suspension (12.5, 6.25, 3.125 g/kg), respectively, and the rats in group group C, D and E were given semiaquilegia 12.5, 6.25 and 3.125 g/kg (0.5 ml administration by gavage), and the rats in group F were administered with 0.104 mg/kg progynova, while the rats in group A and B were administered with same amount of normal saline. The female and male rats in each group were caged at a ratio of 2:1. On the 5th and 8th day of pregnancy, 8 rats were randomly selected from each group. After anesthesia, abdominal aortic blood of rats was collected, and their uterus was striped. The levels of serum estrogen (E2) and progesterone (PROG) of these rats were detected. HE staining was applied to observe morphological changes of endometrium tissue and vascular distribution. The immunohistochemistry was used to detect the expression levels of VEGF and ITG-β3. The number of pregnant rats and number of implantation points were calculated. Results: On the 5th day of pregnancy, the change of endometrial lesions of rats in group B, C, D, E and F was significant more than that of rats in group A, and the number of blood vessels, the levels of serum E2 and PROG, expression levels of VEGF and ITG-β3 in endometrium tissue of rats in group B, C, D, E and F were significant less. On the 5th day of pregnancy, the change of endometrial lesions of rats in group C, D, E and F was significant more than that of rats in group B, and the number of blood vessels, the levels of serum E2 and PROG, expression levels of VEGF and ITG-β3 in endometrium tissue of rats in group C, D, E and F were significant higher.On the 8th day of pregnancy, the average number of implantation blastocysts of rats in group B, C, D, E and F was significant less than that of rats in group A, but that of rats in group C, D, E and F was significant more than that of rats in group B (P<0.05). Conclusion: Semiaquilegia granule can increase number of implantation blastocysts of rats with bad endometrial receptivity because of kidney-deficiency blood-stasis by improving their levels of serum sex hormones and VEGF and ITG-β3 expression, and promoting the blood supply of their endometrium.
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