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| Expressions and clinical significances of LncRNA NEAT1 and HOTAIR in exfoliated cells of patients with cervical persistent HPV infection |
| Baoshan District Dachang Hospital, Shanghai, 200436 |
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Abstract Objective: To investigate the expression of long non-coding RNA (LncRNA) nuclear enrichment transcript 1 (NEAT1), and HOX transcriptional antisense RNA (HOTAIR) in exfoliated cells of patients with cervical persistent high-risk human papillomavirus (HPV) infection, and to explore their clinical significances. Methods: 56 patients with cervical HPV infection were selected from August 2017 to August 2018, and their exfoliated cervical cells were collected for liquid-based thinprep cytologic test (TCT) and HPV DNA genotyping detection. According to the results of HPV detection, the patients were divided into group A (patients with cervical persistent HPV infection) and group B (patients with cervical HPV infection disappeared). The positive expressions of p16 and Ki67 were detected by immunohistochemistry, the expression levels of LncRNA NEAT1 and HOTAIR were detected by qRT-PCR. Spearman analysis was used for abalyze the correlations, and ROC was used for study the diagnostic values. Results: 14 high-risk subtypes HPV and 5 low-risk subtypes were found in 56 samples by conduction hybridization method. The relative expression levels of LncRNA NEAT1 and HOTAIR detected by qRT-PCR in group A were significant higher than those in group B, and the positive expressions of p16 and Ki67 detected by immunohistochemistry in group A were also significant higher than those in group B (P<0.05). LncRNA NEAT1 level was positively correlated with p16 and Ki67 levels (P=0.033, 0.027), and HOTAIR level was also positively correlated with p16 and Ki67 levels (P=0.021, 0.013). The AUC areas, the specificity, and the sensitivity of LncRNA NEAT1 level for diagnosing cervical persistent HPV infection were 0.838, 95.65, and 60.0, respectively. And the AUC areas, the specificity, and the sensitivity of level of HOTAIR for diagnosing cervical persistent HPV infection were 0.936, 84.62, and 90.00, respectively. Conclusion: The expressions of LncRNA NEAT1 and HOTAIR in exfoliated cells of patients with cervical persistent HPV infection are abnormally increased, which is positively correlated with the expression levels of p16 and Ki67, so LncRNA NEAT1 and HOTAIR levels maybe used as indicators for diagnosing patients with persistent HPV infection, which maybe used as early screening indicators for cervical cancer.
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