|
|
|
| The effect of circRNA Amotl-1 on preterm pregnant women with gestational diabetes mellitus and its correlation to endothelial colony forming-cells |
| Wenzhou Central Hospital, Zhejiang Province, 325000 |
|
|
|
|
Abstract Objective: To explore the effect of circRNA Amotl-1 on pregnant women with gestational diabetes mellitus (GDM) and preterm delivery, and to investigate the relationship between circRNA Amotl-1 and endothelial colony-forming cells. Methods: 60 pregnant women with GDM and preterm delivery were included in study group, and the 10 health pregnant women were included in control group. The endothelial colony-forming cells of women in both groups were collected. The relationship between circRNA Amotl-1 and and endothelial colony-forming cells was observed by scratch test and tubule formation test. Results: The rate of endothelial clonogenic cells of women in the study group was 99.2%, which was signifficant higher than that (35.7%) of women in the control group. In the tubule form experiment, the length of tubule of cell after si RNA transfection was 105.17±1.34m, and that after plasmid transfection was 151.62±2.51m, which were all significant more than that (98.56±3.71m) of cell with blank control (P<0.05). In the cell scratch plane migration experiment, the migration rate of circRNA Amotl-1 plasmid was nearly 100%, that of si RNA was 55.9%, and that in the control group was 5.2%. Conclusion: circRNA Amotl-1 can regulate the occurrence and development of the preterm delivery of pregnant women with GDM by promoting endothelial colony-forming cells.
|
|
|
|
|
|
|
|
|
