To investigate the value of serum progesterone (P) and HCG levels for predicting early spontaneous abortion of women with recurrent spontaneous abortion (RSA), and to analyze the correlation between P and HCG levels and effect of immunotherapy. Methods: 209 pregnant women from May 30, 2017 to December 30, 2018 were collected retrospectively, which included 125 women without abortion history were in group A and 84 women with RSA were in group B. And in this pregnancy, the women in group B during the first trimester of pregnancy were also divided into 35 women with abortion in group B1 and 49 women without abortion abortion in group B2. The P and HCG levels of women during the first trimester of pregnancy (4, 6, 8, 10, 12 gestational weeks) were observed, and the value of P and HCG levels for predicting early pregnancy abortion of women in group B was analyzed. Immunotherapy was performed for the women in group B, and the correlation between P and HCG levels and immunotherapy effect was observed. Results: The number of spontaneous abortion of pregnancy of women in group B were significant higher than those of women in group A (P<0.05). During 4, 6, 8, 10, 12 gestational weeks, the HCG or P levels of women in group B1 was the lowest, and that in group A was the highest (P<0.05). ROC curve analysis showed that HCG level or P level of pregnant women during 8 gestational weeks had predictive value for early abortion in group B (P<0.05), and HCG level combined with P level had higher predicted value for early abortion of women with RSA during re-pregnancy (AUC=0.81 and the sensitivity= 81.5%). A total of 15 women in group B had successful immunotherapy, and the levels of HCG and P of women in group B2 were significant higher than those women in group B1 (P<0.05). Conclusion: The value of P level combined with HCG level of women for predicting the women with RSA is high, both the P level and HCG level are associated with the immunotherapy effects.