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| Expression of long non-coding RNA SNHG5 in ovarian cancer tissues and its effect on autophagy of ovarian cancer cells |
| Chongming Branch, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 202150 |
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Abstract To observe the expression of long non-coding RNA (lncRNA) nucleolar small RNA host gene 5 (SNHG5) in ovarian cancer tissues, and to analyze the effect of SNHG5 expression on autophagy of ovarian cancer cells. Methods: 73 women with ovarian cancer were selected in group A and 82 women with ovarian cyst were selected in group B from August 2018 to March 2019. The expression of SNHG5 in ovarian tissues of women in the two groups was detected by real-time fluorescence quantitative PCR (qRT-PCR), and the relationships between the expression of SNHG5 in ovarian cancer tissue and clinical pathology of women in group A was analyzed. SKOV3 cells were cultured as blank control group, and SKOV3 cells transfected with empty plasmid pcDNA3.1 (+) and recombinant plasmid pcDNA3.1-SNHG5 by Lipofectamine 2000 reagent were in negative control group and SNHG5 over expression group respectively. Cell counting kit (CCK8) method was used to detect the proliferation of SKOV3 cells in each group, the autophagy level of SKOV3 cells in each group was detected by fluoroscopy and electron microscopy, and the expression levels of LC3-I, LC3-II and Beclin 1 protein were detected by immunoblotting (WB). Results: Compared with that (1.13±0.23) in group B, the relative expression (0.49±0.06) of LNC RNA-SNHG5 in group A was significant lower (P<0.05). Compared with that in histological grade G1-G2 and FIGO stage I-II, the percentage of patients with high expression of LNC RNA-SNHG5 in histological grade G3 and FIGO stage III-IV was significant higher (P<0.05). Compared with that (1.03±0.20) in blank control group and that (1.05±0.21) in negative control group, the relative expression of SNHG5 in SNHG5 over expression group (1.97±0.37) had increased significantly (P<0.05). The inhibition rates of SKOV3 cell proliferation in SNHG5 over expression group at 48h, 72h and 96h after transfection were 23.64±4.73%, 43.57±8.71%, and 57.82±11.56%, respectively, which were significant higher than those in blank and negative control group (P< 0.05). Compared with those in the blank group or the negative control group, the proportion of MDC positive cells in SNHG5 over expression group (42.38±7.64) was significant higher, the expression of LC3-I protein in SNHG5 over expression group (0.16±0.03) was significant lower, and the expression of LC3-II or Beclin 1 proteins (0.86±0.18 or 0.97±0.18) was significant higher (all P<0.05). Conclusion: The expression of lncRNA-SNHG5 in ovarian cancer tissues is low and the over expression of lncRNA-SNHG5 significantly inhibits the viability of human ovarian cancer cells and promotes autophagy of cells.
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