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| The value of serum micro RNA 181b and micro RNA 210 levels detected for diagnosing women with hypertensive disorder complicating pregnancy |
| Fengnan District Hospital, Tangshan, Hebei Province, 063300 |
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Abstract Objective: To explore the value of serum micro RNA 181b (miR 181b) and micro RNA 210 (miR 210) levels for diagnosing women with hypertensive disorder complicating pregnancy. Methods: The blood samples from 96 women with hypertensive disorder complicating pregnancy (in study group) and 96 healthy pregnant women (in control group) were collected. Real time fluorescence quantitative PCR (qRT PCR) was used to detect the expression of miR 181b and miR 210. The relationship between the levels of miR 181b and miR 210 and clinical indicators were observed and analyzed. Receiver operating characteristic (ROC) curve was used to evaluate the optimal threshold value of miR 181b or miR 210 for diagnosing hypertensive disorder complicating pregnancy and for evaluating their diagnosis efficiency. Results: The levels of serum miR 181b and miR 210 of women in the study group were significant higher than those of women in the control group (P<0.05). In the study group, the level of serum miR 181b or miR 210 was positively correlated with the levels of serum homocysteine (Hcy), C reactive protein (CRP), and malondialdehyde (MDA) (P<0.05), was negatively correlated with total antioxidant capacity (TAC) and superoxide dismutase (SOD) (P<0.05), but was no correlated with glutathione peroxidase (GSH Px) (P>0.05). ROC curve analysis showed that the cut off value, the sensitivity, and the specificity of miR 181b level were 1.50, 57.3%, and 76.0%, respectively. And the cut off value, the sensitivity and the specificity of miR 210 level was 1.10, 55.2%, and 75.3%, respectively. The accuracy, the sensitivity, and the specificity of miR 181b level combined with miR 210 level were 83.3%, 88.5%, and 85.9%, respectively, which were significant higher than those of miR 181b level or miR 210 level only (P<0.05). Conclusion: The levels of serum miR 181b and miR 210 of women with hypertensive disorder complicating pregnancy increase, and the miR 181b level combined with the miR 210 level can improve the diagnosis efficiency of hypertensive disorder complicating pregnancy.
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