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| Cytogenetic etiological analysis for women with missed abortion and the clinical significance of the biochemical indicators |
| Lianyungang Maternal and Child Health Care Hospital, Jiangsu Province, 222000 |
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Abstract Objective: To explore the cytogenetic etiological analysis of the villi of women with missed abortion, and to analyze its clinical significance. Methods: From January 2015 to March 2017, 98 women with missed abortion were selected into the study group, and another 98 normal pregnant women who voluntarily terminated pregnancy during the same period were selected into the control group. The villus tissues of women in the two groups were collected and the cells were isolated from these villus tissue for cultured. G-banding technique was used to analyze the karyotype of the villus cells, and Immunohistochemical technique was used to detect the expression of MMP-9, TSP-1 and protein grading in villi. Results: The karyotypes of villi cell in the control group were 44+XX or 44+XY, but for karyotypes analysis, 42 cases in 98 villi samples from the study group were failed. Among the 56 villi samples successfully completed karyotypes analysis, there were 6 cases with monomer-X chromosome, 1 case with monomer normal chromosome, 35 cases with trisomy, 7 cases with chimerism, and 6 cases with chromosome deletion or duplication. The rate of positive MMP-9 of villi in the control group was significant higher than that of villi in the study group, while the expression of TSP-1 of the villus tissue in the study group was significant lower than that in the control group (P<0.05). Conclusion: Cytogenetic etiological analysis of the villi of women with missed abortion can provide guidance for the next pregnancy. The expression of MMP-9, TSP-1 of villi can be used as auxiliary diagnostic index for missed abortion.
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