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| The establishment of 8 hydroxydeoxyguanosine detection by a high specificity ELISA assay, and the comparative study of its used in gestational diabetes mellitus |
| 1.Graduate School of Peking Union Medical College, Beijing, 100730；2.National Research Institute for Family Panning |
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Abstract Objective: To establish a method for the detection of 8-OHdG by biotin avidin sandwich antibody ELISA, and to comparison study the level of 8 OHdG levels of patients with gestational diabetes mellitus (GDM). Methods: The 8 OHdG-mcKLH complex was formed by conjugation and used as immunogen to obtain the highly specific paired antibodies 1D4 and 3B8 of 8 OHdG. A biotin avidin labeled double antibody sandwich ELISA was developed for the quantitative detection of 8 OHdG. The method was used for detecting serum 8 OHdG levels of patients with GDMand healthy pregnant women. Results: The coefficient of variation intraassay and between the assays was less than 15%, which had good stabilities, high accuracy and precision. The recovery rates were 99.2%, 100.8% and 94.8%. Serum 8 OHdG level of patients with GDM was (7.3±1.3)ng/ml, which was significant higher than that ［(2.2±0.7) ng/ml］ of normal pregnant women (P<0.001). Conclusion: The level of serum 8 OHdG of patients with GDM may be closely related to the occurrence of GDM and the progression of the disease. The method of detecting 8 OHdG in this study have provide more accurate and convenient test method for studying the relationship between oxidative damage and disease.
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