To investigate the expression characteristics of serum apelin and neutrophil gelatinase associated lipocalin-2 (lipocalin-2) of pregnant women with hypothyroidism, to analyze the relationship between the expressions of serum apelin and lipocalin-2 and dyslipidemia, and to study their influence on the pregnancy outcomes. Methods: 92 pregnant women with subclinical hypothyroidism were selected in study group, and 60 normal pregnant women hypothyroidism women were selected in control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect serum apelin and lipocalin-2 levels, and automated biochemical analyzers was used to test the levels of serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), apolipoprotein A (ApoA), and Apolipoprotein B (ApoB) .The relationship between the serum apelin and lipocalin-2 and blood lipids levels, and the levels of apelin and lipocalin-2 influenced on the pregnancy outcomes were analyzed by Pearson correlation analysis. Results: The serum levels of TC, TG and HOMA-IR of women in the study group were significant higher than those of women in the control group (P<0.05), LDL level was significant lower than that of women the control group (P<0.05), but there were no significant different levels of HDL the ApoA and ApoB between the two groups (P>0.05). The levels of serum apelin and lipocalin-2 of women in study group were significant higher than those of women in control group (P<0.05). Correlation analysis showed that apelin and lipocalin-2 were positively correlated with levels of TC, TG and HOMA-IR (r=0.419, 0.435, 0.437, 0.357, 0.521, 0.603, P<0.05), and negatively correlated with LDL level (r=-0.406,-0.395, P<0.05), but there were no correlated to the levels of HDL, APoA, ApoB (P>0.05). There were no significant different in the incidence of neonatal hypothyroidism, fetal growth restriction, gestational diabetes mellitus, gestational hypertension, placental abruption, fetal death, and abortion between the two groups (P>0.05). However, the rate of low birth weight infants in study group was significant higher than that in control group (P<0.05). Conclusion: As for pregnant women with hypothyroidism, the levels of Apelin and lipocalin-2 are correlated to dyslipidemia, and maybe related to the rate of low birth weight infants.