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| CRISPR/Cas9 system and its application in non-coding RNA editing |
| 1. Reproductive & Genetic Center of National Research Institute for Family Planning, Beijing, 100081; 2. Graduate School, Peking Union Medical College |
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Abstract modification is one of the most important means for studying the function of genes. The development of genetic modification technique has undergone a process from gene targeting to the third generation of artificial endonuclease. Recently, the third generation genetic engineering tool called Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system has been widely applied to biomedical research due to its simple operation, low cost and high efficiency. Noncoding RNAs (ncRNAs) have no protein-coding capability and they participate in almost all physiological and pathological processes of the form of RNA directly. This article briefly reviews the structure, development and application of CRISPR/Cas9 technology, and then highlights its application in the editing of miRNAs and lncRNAs.
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