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| Correlation analysis of the expression of miR 106b and DAB2 in endometrial carcinoma tissue with clinical pathology and prognosis of patients with endometrial carcinoma |
| 1.Medical College of Nanjing University, Nanjing, Jiangsu, 212400；2.Nanjing Drum Tower Hospital |
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Abstract Objective: To investigate the relationships between the expressions of micro RNA106b(miR 106b) or Disabled homolog 2 (DAB2) in endometrial carcinoma tissue and clinical pathological features and prognosis of patients with endometrial carcinoma. Methods: The clinical data of 70 patients with endometrial carcinoma who underwent surgical excision( in observation group) from May 2010 to February 2015 in medical college of Nanjing university college were collected, another 70 specimens from the cancerous adjacency tissue were included in normal group at the same period. QRT PCR was used to detect the expressions of miR 106b and DAB2 in endometrial tissue, and the expression of DAB2 protein in endometrial tissue was also detected by immunohistochemistry. According to the expression level of miR 106b, the specimens were also divided into high expression group and low expression group. The relationship between the expressions miR 106b and DAB2 with clinical pathological characteristics was observed and analyzed. Pearson method was used to make the correlation analysis, Kapla Meier was used to survival analysis, and multifactor analysis was carried out by Cox analysis. Results: The expression of miR 106b in endometrial carcinoma tissue of patients in observation group was significant higher than that of patients in normal group (P< 0.05), while the expression of DAB2 of patients in observation group was significant lower than that of patients in normal group (P< 0.05), and the immunohistochemical results had showed that the positive rate of DAB2 protein in endometrial carcinoma tissue of patients in observation group was significant lower than that of patients in normal group (P< 0.05). Clinical pathological analysis showed that the expressions of miR 106b and DAB2 were significant correlated with staging of endometrial carcinoma by FIGO, and level of P53 and Ki67 (P< 0.05). Pearson correlation analysis had showed that there was negative correlation between miR 106b expression and DAB2 expression (P< 0.05). Kaplan Meier analysis had showed that the level of PFS in miR 106b low expression group was significant higher than that in miR 106b high expression group (P < 0.05), and the level of PFS in DAB2 positive expression group was also significant higher than that in DAB2 negative expression group (P < 0.05). Cox multiple regression analysis had showed that the expressions of miR 106b and DAB2 were risk factors for adverse prognosis of patients. Conclusion: In endometrial carcinoma tissue of patients, the expression of MiR 106b increases, but the expression of DAB2 decreases, which are negatively correlated between the expression of MiR 106b and DAB2. And the expression of MiR 106b and DAB2 are all related to tumor tissue differentiation, and tumor cell proliferation and migration, so it can be used as an indicator for early diagnosis and evaluation of prognosis of patients with endometrial carcinoma.
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