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| Study of the expression of endocannabinoid system in SD rats model with PCOS |
| 1. The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000; 2. National Research Institute for Family Planning |
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Abstract Objective: To study the expression of endocannabinoid system in the endometrium of rats with PCOS. Methods: SD rats were fed letrozole to make rats model with PCOS. The body weight, estrus cycle, weight changes of uterus and ovarian of rats were observed. Real time PCR was used to detect the expression of endocannabinoid system, such as CB1, CB2, FAAH and NAPEPLD mRNA. Results: The prolonged time of administration of letrozole had a significant effect on increasing the weight of rats with PCOS when compared to rats in control group. After 21 days, compared with those in control group, the weight of body and ovary of rats with PCOS increased significantly, but the weight of uterus of rats with PCOS was significant lower. And the estrus cycles of rats with PCOS were disordered, the expressions of CB1 and NAPEPLD mRNA increased significantly, but the expression of FAAH mRNA decreased significantly. Conclusion: The molecular expressions of endocannabinoid system of rats with PCOS are abnormal, so it provides theoretical basis for further studies on the relationship between abnormalities of endocannabinoid system and the pathogenesis of PCOS or subsequent adverse pregnancy outcomes.
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