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| Effect analysis of laserdrilling artificial shrinkage used to human blastocyst vitrification cryopreservation |
| Reproductive Centre of Family Planning Research Institute of Guangdong Province, Key Caboratory of Male Reproductive and Genetics of National Health and Family Planning Commission of China,Guangzhou |
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Abstract Objective: To investigate clinical effect of laser drilling-artificial shrinkage (LDAS) of blastocoels before blastocysts vitrification cryopreservation. Methods: The human blastocysts free-thawed blastocyst cycles (FTB) were performed with laser-drilling shrinkage before vitrified cryopreservation (shrinkage group) or without laser-drilling shrinkage before vitrified cryopreservation (control group). The outcomes were compared between the two groups. Results: All the frozenthawed blastocysts in two groups had been embryos transferred in clinic, which included total 317 FTB cycles. 178 FTB cycles have been taken in control group, and other 139 FTB cycles had been taken in shrinkage group. the age of patients, the years of infertility, endometrium thickness on ovulation day, average number of the embryos transferred, high quality blastocyst rate, abortion rate, multiple pregnancy rate and ectopic pregnancy rate had no statistical difference in the two groups (P>0.05). The clinical pregnancy rate (44.9%) and the implantation rate (34.4%) in control group were significant lower than those in shrinkage group (66.9% and 48.5%, respectively) (P=0.001). Conclusion: The clinical pregnancy rate and implantation rate of FTB are significantly improved by LDAS of blastocoels before vitrified cryopreservation, which is worthy of clinical exploration and application.
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