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| Correlation study between markers of macromolecular oxidative damage in seminal plasma and semen parameters |
| 1. Department of Epidemiology and Biostatistics, School of Public Health, Peking University; Institute of Reproductive & Child Health, School of Public Health; Key Laboratory of Reproductive Health, Ministry of Health, Beijing, 100191；2.Beijing Obstetrics and Gynecology Hospital, Capital Medical University |
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Abstract To investigate the relationship between macromolecular oxidative damage in seminal plasma and semen parameters. Methods: A total of 96 men who came to seek infertility treatment were recruited as subjects. A facetoface questionnaire was administered by the trained investigators. Semen samples were collected by masturbation. Routine semen parameters were determined by computeraided sperm analysis system. Protein oxidative damage and lipid oxidative damage were showed by the concentration of protein carbonyl derivatives (PC) and 8isoprostaglandin F2α (8isoPGF2α), respectively, and the seminal plasma was measured by enzymelinked immunosorbent assay. To examine the association of semen parameters with oxidative damage indexes, all subjects were divided into normal group and abnormal group according to the normal range of sperm concentration, progressive motility rate and teratozoo spermia rate. Results: 8isoPGF2αconcentration of subjects in abnormal sperm concentration group was higher than that of subjects in normal sperm concentration group (P<0.05). There was no significant difference in PC concentration between normal sperm concentration group and abnormal sperm concentration group. No significant differences were found in PCand 8isoPGF2αconcentration between normal and abnormal groups in term of progressive motility rate and teratozoospermia rate. Pearson correlation analysis showed that 8isoPGF2α concentration was negatively correlated with sperm concentration (r=0.254, P=0.018), but uncorrelated to other sperm parameters; PC concentration was not significantly correlated with any semen parameters. Multiplelinear regression analysis showed that sperm concentration decreased 7×104/ml by1 ng /ml increase of 8isoPGF2α. Conclusion: The decrease of sperm concentration may be caused by lipid oxidative damage in men.
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