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| Experimental study of spermicidal and sperm agglutinated efficacy of red kidney bean lectin gel |
| Key Lab of Yunnan Province for Biomass Energy and Environmental Biotechnology, Engineering Research Center Of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, School of Life Sciences, Yunnan Normal University, Kunming, Yunnan, 650500 |
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Abstract To explore the spermicidal and sperm agglutinated efficacy of red kidney bean lectin (RKBL). Methods: ① Sander Cramer test has been used to observe the spermicidal efficacy when mixed 5, 1, 0.5, 0.25, 2.5, 01, 0.05, 0.025, 0.01, 0.005, 0.0025, 0.001 mg/ml of RKBL gels with the same volume of semen in 20s, and the lowest dose of RKBL which killed all sperm in 20s was also explored, and the effective concentration of RKBL on inducing sperm agglutination was also observed. ②5, 1, 0.5, 0.25, 2.5, 0.1, 0.05, 0.025, 0.01, 0.005, 0.0025, 0.001 mg/ml of RKBL gels were put into the bottom of the “V” pipe, then added equal volume HTF medium to both side of the “V” type pipe. After that, Sperm was added into one side of the pipe, and then the effect of stopping sperm penetration and killed sperm were observed. Results: when the RKBL gel concentration was 1mg/ml or above, sperm was inactivation and instant death, so the lowest dose for effective killing sperm by the Sander Cramer test in 20s was 1mg/ml. Sperm agglutination had occurred when RKBL gel concentration was 0.01mg/ml or above. In “V” type pipe, different concentrations of RKBL gel could prevent sperm penetration. When the concentration of RKBL gel was 1mg/ml or above, the sperm completely lost activity near the gel. Conclusion: The lowest effective concentration of RKBL gel for killing sperm is 1mg/ml or above. Some low concentration of RBKL gel can induce sperm aggregate in different degrees. The different
DOI:10.3969/j.issn.10048189.2017.10.002
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