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Abstract Objective: To explore the immature testis allotransplantation model in the mouse using the same strain ICR mouse as a recipient, and to make a improvement based on the previous reports. Methods: The donor was the immature testis of six-day ICR-mouse. The recipient was the same strain ICR mouse. The immature testis of ICR mice were transplanted into the back of the neck in the recipient mice. After 3, 4, 5 weeks, the status and the volume of the allograft were observed, and then the histopathology of the allograft was assessed. The level of TESK1 mRNA expression was analyzed by Q-PCR. Results: The volume of the allograft increased after allotransplantation. The average volume was (17.3±2.6) mm3 before transplantation, (203.1±14.3) mm3 after 3 weeks of transplantation, (236.4± 27.5) mm3 after 4 weeks of transplantation, (220.9±17.4) mm3 after 4 weeks of transplantation. Histopathologic results showed that seminiferous tubules developed well and the spermatogenic function recovered after immature testis allotransplantation. No significant differences of seminiferous tubules intact conditions were found among three groups after allotransplantation (P>0.05). The expression level of TESK1 mRNA increased after transplantation. The relative expression of TESK1 mRNA was (1.19±0.1) times before transplantation, (6.74±0.8) times in 3-week allograft, and (11.23±1.2) times in 5-week allograft the number of the control group (P<0.05). Conclusion: It is feasible that ICR mouse immature testis were allotransplanted using the same strain ICR mouse as a recipient.
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