To explore the efficacy of Baofukang suppository as adjuvant method of the recombinant human interferon α-2b gel for treating patients with high-risk human papillomavirus (HR-HPV) infection, and to study its effect on HPV viral load and the cervicitis of the patients. Methods: 100 patients with HR-HPV infection were selected and were randomly divided into two groups (50 cases in each group) from January 2022 to February 2023. The patients in the two groups were treated with the recombinant human interferon α-2b gel, and the patients in the observation group were given Baofukang suppository as adjuvant treatment additionally. The clinical efficacy, the vaginal secretion cleanliness degree, the Nugent score, the HPV load, the cervical inflammation score and the levels of serum inflammatory factors, such as tumor necrosis factor-α(TNF-α) and transforming growth factor-β1 (TGF-β1), of the patients were compared between the two groups. Results: After treatment, the total clinical effective rate (94.0%) of the patients in the observation group was significantly higher than that (74.0%) of the patients in the control group. The vaginal secretion cleanliness degree (1.02±0.28), the Nugent score (2.19±0.37) and the RLU/CO of HR-HPV load ［4.37 (0.08, 2162.75)］ of the patients in the observation group were significantly lower than those (1.75±0.32, 2.84±0.50 and 78.63 (1.03, 2633.41) of the patients in the control group. The cervical inflammation score (10.15±1.16 points), the levels of serum TNF-α (5.83±1.52 ng/L) and TGF-β1 (16.37±2.41 ng/L) of the patients in the observation group were significantly lower than those (17.43±1.55 points, 11.49±2.58 ng/L and 21.56±2.67 ng/L) of the patients in the control group (all P<0.05). There was no significant difference in the total incidence of adverse reactions (10.0% vs. 20.0%) of the patients between the two groups (P>0.05). Conclusion: Baofukang suppository combined with recombinant human interferon α-2b gel for treating the patients with HR-HPV infection can increase the effectiveness, which can effectively improve the vaginal cleanliness, reduce the HPV load and relieve the cervical inflammation of the patients, and without increase of the adverse reactions.