To investigate the predictive value of serum extracellular matrix protein-3 (Fibulin-3) and angiopoietin-2 (Ang2) expression levels for placental abruption of pregnant women with severe preeclampsia. Methods: A total of 380 pregnant women with severe preeclampsia were selected in research group and were divided into group A (50 cases with placental abruption) and the group B (330 cases without placental abruption) according to occurrence of placental abruption from January 2019 to December 2019. 200 healthy pregnant women who came to hospital for antenatal examination were selected in control group during the same period. The expression levels of serum Fibulin-3 and Ang2 of the women were compared between the two groups. Receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of the serum Fibulin-3 and Ang2 levels of the women for their placental abruption. Multivariate logistic regression method was used to analyze the related factors of placental abruption of the women with severe preeclampsia. Results: The levels of serum Fibulin3 (33.25±7.52 ng/ml) and Ang2 (6.22±1.42 ng/ml) of the women in the research group were significantly lower than those (98.62±10.47 ng/ml and 17.22±5.20 ng/ml) of the women in the control group, and which of the women in group A were significantly lower than those of the women in group B (P＜0.05). ROC curve analysis showed that the area under the curve, the cut-off value, the sensitivity, and the specificity of the Fibulin-3 level of the women with severe preeclampsia for diagnosing their placental abruption were 0.769, 25.35ng/L, 82.6%, and 75.8%, respectively. The area under the curve, the cut-off value, the sensitivity, and the specificity of the Ang2 level of the women with severe preeclampsia for diagnosing their placental abruption were 0.785, 5.52ng/L, 85.4%, and 78.0%, respectively. Multivariate analysis showed that the abnormal systolic blood pressure, diastolic blood pressure, albumin, serum Fibulin-3 and Ang2 levels of the women were the risk factors of their severe preeclampsia and placental abruption (P＜0.05). Conclusion: The serum levels of Fibulin-3 and Ang2 of the pregnant women with severe preeclampsia are low abnormally, and which of the women with placental abruption are lower, and both of which are expected to be the effective indicators for early diagnosing the placental abruption of these women.