To investigate the situation of infertility of women from assisted reproductive clinics, and to analyze the risk factors of their infertility. Methods: Retrospective method was used to collect the clinical data of 1016 infertility women (case group) in the assisted reproduction clinic of Tangshan maternal and child health care hospital. According to the ratio of 1:1, the women with natural pregnancy and without contraception used within 1 year were selected in control group. Multivariate logistic regression analysis was used to analyze the related factors affecting female infertility. Results: There were 254 (25.0%) women with primary infertility and 762 (75.0%) women with secondary infertility in the case group. There were no significant differences in age, educational level, history of abortion/induced labor, history of ectopic pregnancy, and history of systemic diseases of the women between the two groups (P＞0.05). The age of marriage, the menarche age, the age and frequency of the first sexual intercourse, the menstrual history, the history of postpartum and  post-abortion complications, the history of genitourinary infection, and the proportion of obesity of the women between the two groups (P＞0.05). Multivariate analysis showed that sexual frequency >8 times/month (OR=1.506, 95%CI: 1.067-2.337), the irregular menstruation (OR=1.412, 95%CI: 1.152-2.196), and the history of postpartum and post-abortion complications (OR=3.585, 95%CI: 1.682-6.824), the history of urogenital infection (OR=4.898, 95%CI: 3.153-13.296), the obesity (OR=2.065, 95%CI: 1.625-6.824) were all the risk factors of female infertility. The menarche <13 years old (OR=0.814, 95%CI: 0.599-0.924) and the age of the first sexual intercourse ≤20 years old (OR=0.892, 95%CI: 0.718-0.987) were the protective factors of female infertility (all P＜0.05). Conclusion: Early menarche age, early sexual life, history of urogenital infection, obesity, and so on of the women are the independent risk factors of their infertility, which suggest that local reproductive health education should be strengthened to reduce the risk factors of infertility, and to decrease the risk of infertility.