To investigate the correlation between the levels of serum miR-214 and connective tissue growth factor (CTGF) of women with intrauterine adhesions (IUA) and their clinicopathological factors and postoperative IUA recurrence. Methods: A total of 80 women with IUA were collected in study group and 60 healthy women without IUA who wanted physical examination were selected in control group from September 2019 to September 2020. qRT-PCR was used to detect the level of serum miR-214, and enzyme-linked immunosorbent (ELISA) method was used to detect the expression level of serum CTGF. Pearson method was used to analyze the correlation between the serum miR-214 level of the women and their CTGF level. The different levels of serum miR-214 and CTGF expressions of the women before and after surgery were analyzed. Receiver operating characteristic (ROC) curve was used to analyze the value of miR-214 and CTGF expression levels for predicting the postoperative recurrence of IUA. Multivariate Logistic regression was used to analyze the risk factors of the postoperative recurrence of IUA. Results: The level of serum miR-214 (0.46±0.08) of the women in the study group was significantly lower than that (1.24±0.16) of the women in control group, and the level of CTGF (141.35±34.18 ng/ml) of the women in the study group was significantly higher than that (66.75±17.48 ng/ml) of the women in the control group (all P＜0.05). The serum miR-214 level of the women was negatively correlated with their CTGF level (r=-0.538, P＜0.05). The levels of serum miR-214 and CTGF of the women in the study group before surgery and had significantly different from those after surgery (P＜0.05). ROC curve analysis showed that the area under the curve, the sensitivity, and the specificity of the serum miR-214 level for predicting postoperative IUA recurrence of the patients were 0.787, 66.7%, and 91.2%, respectively, and which of the serum CTGF level for predicting postoperative IUA recurrence of the patients were 0.823, 75.0%, and  91.2%, respectively. Multivariate analysis showed that the number of induced abortions >3 times, the IUA in muscle, the extent of IUA >2/3 of uterine cavity, the low expression level of miR -214, and the high level of CTGF of the women were the independent risk factors of their postoperative IUA recurrence (P＜0.05). Conclusion: The expression levels of serum miR-214 and CTGF of the women with IUA are abnormal, which has certain value for diagnosing their postoperative IUA recurrence, and are expected to be important indicators for predicting the poor prognosis of IUA.