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| Expression of the mucin 5B level in cervical cancer tissue and its correlation with the E6/E7 mRNA expression of human papilloma virus |
| Shanghai Baoshan Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, 201900 |
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Abstract To investigate the expression of the mucin 5B (MUC5B) level in cervical cancer tissue, and to study its correlation with the E6/E7 mRNA expression of human papilloma virus (HPV). Methods: The clinical data of 212 patients with suspected cervical cancer who had admitted in hospital from June 2018 to June 2021 were collected retrospectively. All these patients had undergone cervical biopsy and were divided into group A (49 cases with cervical intraepithelial neoplasia (CIN) I), group B (50 cases with CIN II), group C (51 cases with CIN III) and group D (62 cases with cervical cancer) according to the pathological results of the patients. The fully automated nucleic acid detection system was used to detect the HPV E6/E7 mRNA expression, and the immunohistochemical method was used to determine the expression of MUC5B in the different cervical cancer tissues. The correlation between the level of MUC5B in the cervical cancer tissue of the patients and their clinicopathological characteristics was analyzed. Spearman method was used to analyze the correlation between the MUC5B level of the patients and their HPV E6/E7 mRNA expression. Kaplan-Meier method was used to analyze the correlation between the expression of MUC5B in cervical cancer tissues of the patients and their survival rate. Results: The positive expression rates of MUC5B and HPV E6 /E7 mRNA of the patients in group A (24.5% and 32.7%), in group B (46.0% and 54.0%), in group C (66.7% and 74.5%) and in group D (77.4% and 82.3%) had increased gradually. The patients with different MUC5B protein expression had the different lymph node metastasis and the different invasion depth of the cancer. The MUC5B expression of the
patients with cervical cancer was positively correlated with their HPV E6/E7 mRNA expression. Kaplan Meier survival curve showed that the cumulative survival rate (86.9%) of the patients with negative MUC5B in 3 years was significantly higher than that (71.7%) of the patients with positive MUC5B (all P<0.05). Conclusion: MUC5B is highly expressed in cervical cancer tissues of the patients, and the expression of MUC5B protein of the patients is positively correlated with their expression of HPV E6/E7 mRNA.
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