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| The expression level of Amphiregulin in peripheral blood and placenta tissue of pregnant women with preeclampsia and its corelation with the pregnancy outcomes |
| Qingdao Women's and Children's Hospital， Shandong Province, 266000 |
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Abstract To investigate the expression level of Amphiregulin in peripheral blood and placenta tissue of patients with preeclampsia and its relationship with their pregnancy outcomes. Methods: A total of 203 pregnant women with preeclampsia were selected as the research objects from June 2019 to September 2020. According to the severity of preeclampsia, these women were divided into group A (102 cases with mild preeclampsia) and group B (101 cases with severe preeclampsia). 108 healthy pregnant women were selected in group C. Real-time fluorescence reverse transcription (RT-PCR), enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were used to determine the expression levels of Amphiregulin mRNA and protein in peripheral blood and placental tissues of the women in the three groups. The pregnancy outcomes (placental weight, newborn birth, body weight, 1min Apgar score) of the women in the three groups were recorded. The differences of the expression of Amphiregulin in peripheral blood and placental tissues of the women were compared among the three groups. The relationship between the expression of Amphiregulin in peripheral blood and placental tissues of the women and their pregnancy outcomes was analyzed. Results: The expression levels of the serum and placental Amphiregulin mRNA and protein, the positive rate of Amphiregulin, the placental weight, the newborn birth weight, and the newborn 1min Apgar score of the women in group B, group A, and group C had increased gradually. The expression levels the mRNA and protein of serum and placental Amphiregulin of the women were positively correlated with their placental weight, newborn birth weight, and 1min Apgar score (all P＜0.05). Conclusion: The expression levels the mRNA and protein of serum and placental Amphiregulin of the women with preeclampsia decrease abnormally, and which will decrease with the severity of preeclampsia. The expression levels the mRNA and protein of serum and placental of the women are negatively correlated with the severity of their preeclampsia, and were positively correlated with their adverse pregnancy outcomes. It is suggested that Amphiregulin is expected to be a biological marker for evaluating the pregnancy outcomes of the pregnant women with preeclampsia.
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