To analyze the effects of anterior quadratus lumborum and pudendal nerve block combined with general anesthesia during ovarian cancer operation of patients on their perioperative stress indicators and prognostic sexual function. Methods: The clinical data of 82 patients undergoing radical resection of ovarian cancer from January 2018 to December 2019 were collected, which included 43 cases with anterior quadratus lumborum and pudendal nerve block combined with general anesthesia in observation group and 39 cases with anterior quadratus lumborum block combined with general anesthesia in control group. The situations of operation and anesthesia-related adverse reactions of the patients in the two groups were recorded. The stress response stutas, such as the levels of cortisol (COR), norepinephrine (NE) of the patients before surgery and in the 1st day after surgery, and the quality of life evaluated by Functional Assessment of Cancer Therapy-Ovary Cancer (FACT-O) of the patients before surgery and in the 6th month after surgery were compared between the two groups. The changes of postoperative sexual life recovery and sexual function of the patients in the two groups were evaluated by Chinese Version of Female Sexual Function Index (CV-FSFI). Results: The intraoperative dosage of sufentanil (10.06±1.89μg) and the postoperative analgesia rate remedied (2.3%) of the patients in the observation group were significantly lower than those (11.53±2.11μg and 20.5%) of the patients in the control group. The time to wake up, the time to get out of bed for the first time, and the time to exhaust for the first time of the patients in the observation group were significantly lower than those of the patients in the control group. The incidence of anaesthesia related adverse reactions (16.3%) of the patients in the observation group was significantly lower than that (38.5%) of the patients in the control group (P<0.05). The levels of COR and NE of the patients in both groups in the 1st day after operation had increased significantly, and which of the patients in the control group were significantly higher than those of the patients in the observation group (P<0.05). There were no significant differences in FACT-O score in different dimensionality, the postoperative sexual recovery time, and the CV-FSFI score of the patients between the two groups (P>0.05). The physiological status and FACT-O score of functional status of the patients in both groups in the 6th month after operation were significantly higher than those before operation, while social/family status and FACt-O scores of emotional status of the patients in both groups in the 6th month after operation were significantly lower. CV-FSFI scores of the patients in both groups in the 12th month after operation were significantly lower than those before operation (all P<0.05). Conclusion: Anterior quadratus lumborum and pudendal nerve block combined with general anesthesia during radical resection of ovarian cancer has good analgesic effect, which can reduce the anesthetic dosage, decrees the risk of adverse reactions, and relieve the postoperative stress response. The decrease of the postoperative sexual function should be paid attention to in clinical practice.