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| Effects of hypothyroidism of pregnant mice on the glucose metabolism of their offspring |
| Qingdao Hospital Affiliated to Shandong First Medical University, Qingdao, Shandong Province, 266000 |
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Abstract To study the effects of hypothyroidism of pregnant mice on the glucose metabolism of their offspring. Methods: In this study, 80 female SPF newborn mice were randomly divided into model group and control group (40 mice in each group). The mice in the model group were fed with 0.02% propyl thiouracil for establishing the model mouse with hypothyroidism, and the mice in the control group were fed with normal drinking water. After these mice were found to have vaginal emboli, they were reared in separate cages for 0, 3, 7, 14, and 21 days, and the blood of these mice were collected from their posterior orbit. The levels of thyroid hormone T4, thyroid stimulating hormone (TSH), and blood glucose of the mice were detected by chemiluminescence method. Results: The T4 level of the mice in the model group before enrolled in this study (D0) and in the 3rd day after intervention (D3) had no significant different from that of the mice in the control group (P>0.05), but which of the mice in the model group in D7, D14, or postpartum (P1) was significantly higher than that of the mice in the control group (P<0.05). The TSH level of the mice in the model group at D0 had no significant different from that of the mice in the control group (P>0.05), but which of the mice in the model group in D3, D7, D14, or P1 was significantly higher than that of the mice in the control group (P<0.05). There were no significant differences in the gestational days and number of babies of the mice between the two groups (P>0.05). The values of gestational weight gain of the mice and the birth weight of the offspring in the model group were significantly lower than those offspring in the control group. The random blood glucose level of the offspring in the model group was significantly higher than that of the offspring in the control group. There was no significant difference in the fasting, or postprandial 15 min or 30 min blood glucose level of the offspring between the two groups (P>0.05). The postprandial 60 min or 120 min blood glucose level of the offspring in the model group was significantly higher than that in the control group (P>0.05). Conclusion: The blood glucose levels of the pregnant mice with hypothyroidism and their offspring are abnormal.
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