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| Influence of umbilical cord-derived mesenchymal stem cells on the angiogenesis of the model of human ovary cortex after xenotransplantation |
The First Central Hospital of Tianjin, Tianjin, 300192 |
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Abstract To investigate the influence of umbilical cord derived mesenchymal stem cells (UC-MSCs) on the angiogenesis of the model of human ovary cortex after xenotransplantation. Methods：One sample of donated ovarian cortex was cryopreserved by vitrification. After resuscitation, this ovarian cortex was subcutaneously transplanted into the back of 20 mice with severe case and immunodeficiency (SCID). These mice were divided into two groups (10 cases in each group). The mice in the study group were given UC-MSCs treatment, and the mice in the control group were not given UC-MSCs treatment. The local vascular reconstruction of the transplanting local of the mice were observed in the 3rd day，the 7th day, and the 14th day after transplantation of human ovary cortex. The follicle survival and apoptosis, and the situations of the microvascular density and microcirculation blood flow of the mice were compared between the two groups. Results：The normal follicular morphology rate of the mice in the study group in the 7th day or in the 14th day after transplantation (50.2% or 45.4%) was significantly higher than that (38.5% or 34.0%) of the mice in the control group (P<0.05). The proportion of apoptotic follicles in the ovarian tissue of the mice in the study group in the 7th day or in the 14th day after transplantation (19.0% or 31.5%) was significantly lower than that (27.2% or 46.3%) of the mice in the control group (P<0.05). The microvascular density of the mice in the study group in the 3rd day, the 7th day, or the 14th day after transplantation was significantly higher than that of the mice in the control group (P<0.05). The value of local perfusion of blood flow of the transplanting locals of the mice in the study group in the 7th day or in the 14th day after transplantation (106.4± 12.2PU or 124.5± 18.4PU) was significantly higher than that (87.8± 11.9PU or 99.3± 16.6PU) of the mice in the control group (P<0.05). Conclusion：UC-MSCs can accelerate the formation of microvessels of the transplanting locals and can reduce the proportion of follicle apoptosis after transplantation.
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