To investigate the influence of information-motivation-behavior (IMB) guided intervention combined with preoperative vaginal preparation on the postoperative complications and vaginal stump bacterial infection of patients with cervical cancer after radical operation. Methods: 100 patients with cervical cancer who undergone routine preoperative vaginal preparation from January 2018 to December 2018 were selected in control group, and 100 patients cervical cancer who undergone IMB guided intervention combined with preoperative vaginal preparation from January 2019 to December 2019 were selected in observation group. The situations of complication, bladder function, vaginal stump bacterial infection, and the nursing satisfaction of the patients were compared between the two groups. Results: The rates of urinary tract infection (4.0%), deep vein thrombosis (2.0%), vaginal residual bacterial infection (4.0%) of the patients in the observation group were significantly lower than those (12.0%, 9.0%, and 14.0%) of the patients in the control group, and the residual urine volume (75.89±18.24 ml) of the patients in the observation group was significantly less than that (126.76±19.19 ml) of the patients in the control group. The rate of good bladder function (71.0%) and nursing satisfaction (96.0%) of the patients in the observation group were significantly higher than those (40.0% and 86.0%) of the patients in the control group (all P<0.05). Conclusion: After radical operation of cervical cancer, IMB guided intervention combined with preoperative vaginal preparation can actively mobilize cooperate of the patients, reduce the incidence of complications, and promote postoperative rehabilitation.