|
Abstract To investigate the expression and the significant of miR20a autophagy inflammatory body in endometriosis (EMs) tissue. Methods：The medical records of the patients with EMs from January 2019 to December 2020 were collected. The endometrium tissues of 60 patients with EMs were selected in group A, and 60 normal endometrium tissues of the healthy women were selected in group B. The endometrium tissues of the patients in group A were divided into three groups (20 cases in each group), which included group A1 (EMs), group A2 (lipopolysaccharide (LPS) induced), and group A3 (LPS and miR-20a transfection). The expression level of miR20a in different endometrium tissues was detected by fluorescence quantitative PCR. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of serum interleukin (IL)-6, C-reactive protein (CRP), IL-1β and tumor necrosis factor (TNF)-αin EMs ecotopic cells. The expressions of cysteinyl aspartate specific proteinase-1 (caspase-1) and apotosis -associated particlelike protein (ASC), and autophagy protein (LC3) were detected by Western blotting. Results: The levels of miR20a in endometrium tissues and the IL-1β, TNF-α, and CRP levels in serum in group A were significantly higher than those in group B. The levels of IL-6, TNF-α, IL-1β, and CRP in group A1, group A2, and group A3 had increased gradually (all P<0.05). The expression levels of ASC, Caspase-1, LC3, and Beclin-1 in group A1, group A2, and group A3 had increased gradually (P<0.05). Conclusion: The expression of miR20a in ectopic cells of EMs is up-regulated, which suggests that miR20a may be a prognostic marker for EMs. MiR20a may involve in the occurrence of EMs of the patients by regulating their autophagy-inflammasome.
|
|
|
|
|
|
