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| Influence of the selection of the 3rd day embryos of women based on embryo kinetic parameter combined with morphology during in vitro fertilizationembryo transfer on their pregnancy outcomes |
| Jiaozuo Maternal and Child Health Care Hospital, Henan Province, 454000 |
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Abstract To explore the influence of the selection of the 3rd day (D3) embryos of the women based on embryo kinetic parameter by time-lapse imaging (TLI) technology combined with morphological evaluation (CMA) during in vitro fertilization-embryo transfer (IVF-ET) on their pregnancy outcomes. Methods: A retrospective analysis was conducted in 443 cycles of the women during IVF-ET, which included 213 cycles with TLI technology cultured in study group and 230 cycles with routinely cultured in control group. CMA was used to select the embryos in the two groups. The data and the pregnancy outcomes of the cycles were compared between the two groups. In the study group, there were 288 embryos had been transferred, and the time parameters and cleavage mode of 139 embryos with implantation (in group 1) and of 149 embryos without implantation (in group 2) were analyzed. An embryo selection model was established. 65 cycles with the same condition were selected for hierarchical modeling by TLI embryo dynamic parameters combined with CMA. 53 cycles with the first model were in group A, and 12 cycles with the second model were in group B. The clinical outcomes of the women were compared between the study group and the control group. Results: The normal fertilization rate of the women in the study group was significantly higher than that of the women in the control group (P<0.05). The embryos dynamic parameters after TLI technology cultured, such as t4≤37.0h, t8 ≤57.0h, S2 ≤1.0h, and S3 ≤5.5h, and the normal cleavage pattern of the embryos were closely related to the implantation rate of the embryos. After modeling, the normal fertilization rate, the utilization rate of the embryos, the optimal embryo rate, the pregnancy rate, and the implantation rate of the embryos were significantly higher than those of the embryos in the study group and the control group, while the abortion rate was significantly lower. The high quality blastocyst rate and the clinical pregnancy rate of the embryos in group A were 56.0% and 73.6%, respectively, which were significantly higher than those of the embryos in group B (P<0.05). Conclusion: TLI kinetic parameters combined with CMA are used for assessment, and the preliminarily established hierarchical optimal embryo model can improve the clinical outcomes of D3 embryo transfer.
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