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| The expression and significance of SIX1 and E-cad levels in pathological tissue of endometrial hyperplasia |
| Zhuji People's Hospital, Zhejiang Province, 311800 |
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Abstract To explore the expression and significance of mRNA and protein of sine oculis homeobox homolog 1 (SIX1) and E-cadherin (E-cad) in the endometrial tissue of patients with simple endometrial hyperplasia, with complex endometrial hyperplasia, or with atypical endometrial hyperplasia. Methods: The diseased endometrial tissues were collected from 159 patients with endometrial hyperplasia after curettage as the research objects from December 2019 to March 2021. Among these patients, there were 81 cases with simple endometrial hyperplasia in group A and 78 cases with complex and atypical endometrial hyperplasia in group B. The normal endometrial tissues of 83 patients with endometrial cancer were selected in group C during the same period. Real-time fluorescence quantitative PCR (qRT-PCR) method was used to detect the mRNA levels of SIX1 and E-cad mRNA in endometrial tissues of the patients in the three groups, and immunohistochemical method was used to detect their expression of SIX1 and E-cad proteins in endometrial tissues. Pearson's method and Spearman's method were used to analyze the correlation between SIX1 and E-cad mRNA levels of the patients with endometrial hyperplasia and their protein expression. Results: The SIX1 mRNA level and the positive rate of protein expression of the patients in group B were the highest, followed by those of the patients in group A, and which of the patients in group C were the lowest. The E-cad mRNA level and the positive rate of protein expression of the patients in group B were the lowest, followed by those of the patients in group A, and which of the patients in group C were the highest. The level of SIX1 mRNA and the positive rate of protein expression of the patients in group A were significant higher than those of the patients in group C, and the level of E-cad mRNA and the positive rate of protein expression of the patients in group A were significant lower than those of the patients in group C (P＜0.05). In group A and B, the SIX1 level was negatively correlation with the E-cad mRNA level (r=-0.664, -0.572, P＜0.05), and the SIX1 level was also negatively correlation with the E-cad protein expression level (r=-0.354, -0.424, P＜0.05). Conclusion: The expression level of SIX1 in the endometrial tissue of the patients with endometrial hyperplasia increases, but their expression level of E-cad decreases, which may be related to the pathogenesis of endometrial hyperplasia.
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