|
|
|
| Value of the levels of serum exosomes miR-30, TSH, and sFlt-1 in the diagnosis of preeclampsia |
| Suzhou Kowloon Hospital, Shanghai Jiaotong University School of Medcine, Jiangsu Province, 215028 |
|
|
|
|
Abstract To detect the level of serum exosomes miR-30 of pregnant women with preeclampsia, to study the relationship between the miR-30 level of the women and their levels of thyroid stimulating hormone (TSH) and soluble fms-like tyrosine kinase-1 (sFlt-1), and to analyze the value of serum exosomes miR-30 level for diagnosing preeclampsia. Methods: 50 pregnant women with preeclampsia were selected in study group from January 2018 to June 2020, which included 25 women with mild preeclampsia in study group 1 and 25 women with severe preeclampsia in study group 2. 50 healthy pregnant women with singleton were randomly selected in control group during the same period. The levels of serum exosome miR-30, and TSH and sFlt-1 of the women in the three groups were detected, and the values of the levels of serum exosome miR-30, TSH, and sFlt-1 for diagnosing preeclampsia were analyzed. Results: The miR-30 expression level (3.14±0.25), the TSH level (5.32±0.51 uU/ml), and the sFLT-1 level (5.47±0.48 ng/ml) of the women in the study group 2 were significant higher than those (2.26±0.19, 3.65±0.26 uU/ml, and 2.48±0.37ng/ml) of the women in the study group 1, and the miR-30 expression level, the TSH level, and the sFLT-1 level MiR-30 of the women in the study group were significant higher than those (1.02±0.15, 2.13±0.18 uU/ml, and 1.11±0.22 ng/ml) of the women in the control group. The miR-30 expression level of the women in the study group was positively correlated with their TSH level and sFLT-1 level. The serum exosomes miR-30, TSH, and sFLT-1 levels of the pregnant women with preeclampsia were positively correlated with their values of urine protein and blood pressure (P＜0.05). Logistic multivariate regression analysis showed that the increase of serum exosome miR-30, TSH and sFLT-1 levels were the independent risk factors of preeclampsia early (P＜0.05). The combination of serum exosome miR-30 level and the levels of serum TSH and sFLT-1 had the best diagnostic efficiency for preeclampsia, which’s AUG, sensitivity, and specificity were 0.81, 70.1%, and 64.0%, respectively. Conclusion: The levels of serum exosomes miR-30, TSH, and sFlt-1 are involved in the pathogenesis of preeclampsia, so which may be used as diagnostic markers for preeclampsia.
|
|
|
|
|
|
|
|
|
