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Abstract To investigate the distribution of AZF microdeletion in Y chromosome of males, and to discuss its relevant to the clinical feature. Method: 920 infertility males from outpatient service were collected into experimental group, and another 100 normal males were served in control group. Multiple PCR was used to examine the 15 STS sites in AZFa, AZFb and AZFc regions, and the distributions of microdeletions in these regions were analyzed. Results: In the experimental group, 18 cases (1.96%) with AZF microdeletion, which included 12 cases with AZFa deletion, 4 cases with AZFb deletion, 1 case with AZFc deletion, and 1 case with both AZFb and AZFc deletion. 6 types of microdeletion in AZF were found. No abnormality was found in control group. Among 17 males with AZF microdeletion, 6 cases were diagnosed as aspermia, 9 cases were diagnosed as asthenozoospermia or oligoasthenozoospermia, and 2 cases were diagnosed as necrozoospermia. Conclusion: Multiple types of AZF microdeletion were existed in Y chromosome of infertility males, among which, the proportion of AZFc microdeletion is the highest. So microdeletion detection of chromosome Y should be as routine examination and paid more attention to before diagnosing or treating by artificial assisted reproduction.
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